OLYMPUS TECHNOZONE Vol.62 2004-12

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With microscopes playing so many important roles, they must be
assembled with the utmost care


Real Clone by Michihiko Wakayama
*3––Real Clone by Michihiko Wakayama, published by Shogakukan (2000) The author is Dr. Wakayama's older brother, a former high school science teacher. Wakayama wrote a candid account of the life and work of his brother, who succeeded in cloning mice and in the process paved the way for the establishment of cloning technology. That account won a prize for excellence in the sixth Shogakkan non-fiction awards and was published as a book entitled Real Clone.
Miyazawa: 
I hear that each of the researchers at your laboratory uses his/her own microscope. That's surprising.

Wakayama: 
The first thing that strikes people when they visit my lab is the number of microscopes that we have. As the person who sets them all up, I'm the only one who has noticed the subtle differences between individual microscopes. New researchers at the lab use only the microscope designated for their use, so they adjust their eyes to that microscope. It means that they only know what things look like under that particular microscope. A different microscope changes the view of the cells: that is why I have each researcher use a particular microscope.

Ueda: 
In the book Real Clone (*3), the importance of equipment is mentioned as one of several elements contributing to the success of your experiments. As a manufacturer of microscopes, we are naturally delighted to hear this. It makes us feel that we really are contributing to research. I am sure that people at the frontline of manufacturing would love to hear from users in this way more often.

Wakayama: 
Talking at conferences with people doing nucleus transplants (*4), I find that everyone thinks he or she has the best technique. I wouldn't go so far as to say it's a case of incompetent workmen blaming their tools, but if people insist that microscopes should be made better, to speed up the job for example, it means that they don't have the necessary skills. People with the ability to use microscopes at their will don't complain. That goes for me, too, now (laughs).

Ueda: 
Is there a special knack to handling different microscopes or manipulators?
Wakayama: 
Equipment these days is of a very high quality, so you don't need much of a knack to use it. Once you become accustomed to the equipment, you find that your hands just start to move of their own accord. You don't have to take your eye away from the eyepiece, and you will soon learn to perform tasks such as changing the lenses easily. When it comes to taking advantage of the advanced capabilities of the machine, practice is everything.

Miyazawa: 
Just as microscopes are important in your research, Dr. Wakayama, tools are important to me when I assemble those microscopes. I've always been quite particular about the tools I use, for example modifying my own screwdrivers to use with special machine screws. There's no way that I would lend my tools to anybody. I have a lock on my toolbox (laughs).

Wakayama: 
So I assume other people's tools are no good for you either?

Miyazawa: 
Can't use them. I even take my own tools when sitting the technical proficiency exams.

Ueda: 
What was the first microscope that you built?

Miyazawa: 
When I came to the Ina Plant, first I worked in lens grinding, then on microscope electric circuits. Then I started assembling BX Series (*5) microscopes.

Wakayama: 
How long does it take to assemble a single microscope?

Miyazawa: 
About 16 hours I suppose: one every two days. The completed microscopes are shipped with my name stamped inside the main unit, and I say to each one as it leaves the plant, "Bye now, and don't you dare come back!"
Transplanting nuclei using a micromanipulator
*4––Transplanting nuclei using a micromanipulator (click on image to expand it)


Power BX Series BX51 system microscope
*5––Power BX Series BX51 system microscope
One of the latest models in the BX Series of top-of-the-line system microscopes, boasting high optical performance and flexible scalability for a variety of applications from testing to research.
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